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sublibrary plasmid  (Addgene inc)


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    Addgene inc sublibrary plasmid
    Sublibrary Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sublibrary+plasmid/bio_rxiv__2025__09__15__676433-245-14-16?v=Addgene+inc
    Average 93 stars, based on 7 article reviews
    sublibrary plasmid - by Bioz Stars, 2026-06
    93/100 stars

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    Pairwise MultiSTEP secretion and γ-carboxylation functional score correlations between replicate sorting experiments for FIX <t>sublibrary</t> tiles (n ≥ 3,144). Pearson’s correlation coefficients are shown.
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    Figure 7. The UFMylation pathway contributes to Toxoplasma virulence (A) Rank-ordered plots for in vivo fitness scores from the endomembrane-nucleus <t>sublibrary</t> (left) and the metabolism sublibrary (right). UFMylation-related genes are marked in red. Error bars (gray) represent SEM. (B) Fitness scores of the UFMylation-related genes in Vero cells (in vitro), WT mice (WT), and Ifngr1/ mice (KO) are shown as box plots. Each dot represents fitness scores for individual gRNAs. ***p < 0.001; *p < 0.05 (Wilcoxon rank-sum test). (C) The conserved C-terminal amino acid sequences of the UFM1 homologs from indicated apicomplexan (blue) and model organisms (black). The conserved glycine residue is shown by the arrow. (D) Survival curves of WT mice with intra-footpad infection of 103 tachyzoites of WT (n = 10), DUFM1 (n = 6), DUFM1 + UFM1 (WT) (n = 7), and DUFM1 + UFM1 (G87A) (n = 7). ***p < 0.001; N.S., not significant (log-rank test). (E) Complementations of FLAG-tagged WT and G87A UFM1 protein in DUFM1 parasites. (F) Survival curves of WT (n = 10) or Ifngr1/ (n = 9) mice with intra-footpad infection of 103 tachyzoites of DUFM1 parasites. ***p < 0.001 (log-rank test). See also Figure S6.
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    Figure 7. The UFMylation pathway contributes to Toxoplasma virulence (A) Rank-ordered plots for in vivo fitness scores from the endomembrane-nucleus <t>sublibrary</t> (left) and the metabolism sublibrary (right). UFMylation-related genes are marked in red. Error bars (gray) represent SEM. (B) Fitness scores of the UFMylation-related genes in Vero cells (in vitro), WT mice (WT), and Ifngr1/ mice (KO) are shown as box plots. Each dot represents fitness scores for individual gRNAs. ***p < 0.001; *p < 0.05 (Wilcoxon rank-sum test). (C) The conserved C-terminal amino acid sequences of the UFM1 homologs from indicated apicomplexan (blue) and model organisms (black). The conserved glycine residue is shown by the arrow. (D) Survival curves of WT mice with intra-footpad infection of 103 tachyzoites of WT (n = 10), DUFM1 (n = 6), DUFM1 + UFM1 (WT) (n = 7), and DUFM1 + UFM1 (G87A) (n = 7). ***p < 0.001; N.S., not significant (log-rank test). (E) Complementations of FLAG-tagged WT and G87A UFM1 protein in DUFM1 parasites. (F) Survival curves of WT (n = 10) or Ifngr1/ (n = 9) mice with intra-footpad infection of 103 tachyzoites of DUFM1 parasites. ***p < 0.001 (log-rank test). See also Figure S6.
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    Figure 7. The UFMylation pathway contributes to Toxoplasma virulence (A) Rank-ordered plots for in vivo fitness scores from the endomembrane-nucleus <t>sublibrary</t> (left) and the metabolism sublibrary (right). UFMylation-related genes are marked in red. Error bars (gray) represent SEM. (B) Fitness scores of the UFMylation-related genes in Vero cells (in vitro), WT mice (WT), and Ifngr1/ mice (KO) are shown as box plots. Each dot represents fitness scores for individual gRNAs. ***p < 0.001; *p < 0.05 (Wilcoxon rank-sum test). (C) The conserved C-terminal amino acid sequences of the UFM1 homologs from indicated apicomplexan (blue) and model organisms (black). The conserved glycine residue is shown by the arrow. (D) Survival curves of WT mice with intra-footpad infection of 103 tachyzoites of WT (n = 10), DUFM1 (n = 6), DUFM1 + UFM1 (WT) (n = 7), and DUFM1 + UFM1 (G87A) (n = 7). ***p < 0.001; N.S., not significant (log-rank test). (E) Complementations of FLAG-tagged WT and G87A UFM1 protein in DUFM1 parasites. (F) Survival curves of WT (n = 10) or Ifngr1/ (n = 9) mice with intra-footpad infection of 103 tachyzoites of DUFM1 parasites. ***p < 0.001 (log-rank test). See also Figure S6.
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    Image Search Results


    Pairwise MultiSTEP secretion and γ-carboxylation functional score correlations between replicate sorting experiments for FIX sublibrary tiles (n ≥ 3,144). Pearson’s correlation coefficients are shown.

    Journal: bioRxiv

    Article Title: Multiplex, multimodal mapping of variant effects in secreted proteins

    doi: 10.1101/2024.04.01.587474

    Figure Lengend Snippet: Pairwise MultiSTEP secretion and γ-carboxylation functional score correlations between replicate sorting experiments for FIX sublibrary tiles (n ≥ 3,144). Pearson’s correlation coefficients are shown.

    Article Snippet: To barcode each sublibrary, 1 μg of each sublibrary plasmid was digested at 37°C for 5 hours with NheI-HF and SacI-HF (New England Biolabs), incubated with rSAP for 30 minutes at 37°C, then heat-inactivated at 65°C for 20 minutes.

    Techniques: Functional Assay

    Pairwise MultiSTEP secretion and γ-carboxylation functional score correlations for variants that are shared between sublibrary tiles (top row: n = 397, bottom row: n = 380). Pearson’s correlation coefficients are shown.

    Journal: bioRxiv

    Article Title: Multiplex, multimodal mapping of variant effects in secreted proteins

    doi: 10.1101/2024.04.01.587474

    Figure Lengend Snippet: Pairwise MultiSTEP secretion and γ-carboxylation functional score correlations for variants that are shared between sublibrary tiles (top row: n = 397, bottom row: n = 380). Pearson’s correlation coefficients are shown.

    Article Snippet: To barcode each sublibrary, 1 μg of each sublibrary plasmid was digested at 37°C for 5 hours with NheI-HF and SacI-HF (New England Biolabs), incubated with rSAP for 30 minutes at 37°C, then heat-inactivated at 65°C for 20 minutes.

    Techniques: Functional Assay

    Figure 7. The UFMylation pathway contributes to Toxoplasma virulence (A) Rank-ordered plots for in vivo fitness scores from the endomembrane-nucleus sublibrary (left) and the metabolism sublibrary (right). UFMylation-related genes are marked in red. Error bars (gray) represent SEM. (B) Fitness scores of the UFMylation-related genes in Vero cells (in vitro), WT mice (WT), and Ifngr1/ mice (KO) are shown as box plots. Each dot represents fitness scores for individual gRNAs. ***p < 0.001; *p < 0.05 (Wilcoxon rank-sum test). (C) The conserved C-terminal amino acid sequences of the UFM1 homologs from indicated apicomplexan (blue) and model organisms (black). The conserved glycine residue is shown by the arrow. (D) Survival curves of WT mice with intra-footpad infection of 103 tachyzoites of WT (n = 10), DUFM1 (n = 6), DUFM1 + UFM1 (WT) (n = 7), and DUFM1 + UFM1 (G87A) (n = 7). ***p < 0.001; N.S., not significant (log-rank test). (E) Complementations of FLAG-tagged WT and G87A UFM1 protein in DUFM1 parasites. (F) Survival curves of WT (n = 10) or Ifngr1/ (n = 9) mice with intra-footpad infection of 103 tachyzoites of DUFM1 parasites. ***p < 0.001 (log-rank test). See also Figure S6.

    Journal: Cell reports

    Article Title: Host genetics highlights IFN-γ-dependent Toxoplasma genes encoding secreted and non-secreted virulence factors in in vivo CRISPR screens.

    doi: 10.1016/j.celrep.2023.112592

    Figure Lengend Snippet: Figure 7. The UFMylation pathway contributes to Toxoplasma virulence (A) Rank-ordered plots for in vivo fitness scores from the endomembrane-nucleus sublibrary (left) and the metabolism sublibrary (right). UFMylation-related genes are marked in red. Error bars (gray) represent SEM. (B) Fitness scores of the UFMylation-related genes in Vero cells (in vitro), WT mice (WT), and Ifngr1/ mice (KO) are shown as box plots. Each dot represents fitness scores for individual gRNAs. ***p < 0.001; *p < 0.05 (Wilcoxon rank-sum test). (C) The conserved C-terminal amino acid sequences of the UFM1 homologs from indicated apicomplexan (blue) and model organisms (black). The conserved glycine residue is shown by the arrow. (D) Survival curves of WT mice with intra-footpad infection of 103 tachyzoites of WT (n = 10), DUFM1 (n = 6), DUFM1 + UFM1 (WT) (n = 7), and DUFM1 + UFM1 (G87A) (n = 7). ***p < 0.001; N.S., not significant (log-rank test). (E) Complementations of FLAG-tagged WT and G87A UFM1 protein in DUFM1 parasites. (F) Survival curves of WT (n = 10) or Ifngr1/ (n = 9) mice with intra-footpad infection of 103 tachyzoites of DUFM1 parasites. ***p < 0.001 (log-rank test). See also Figure S6.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER T. gondii: Strain RH/Dhxgprt/Dku80/DTGGT1_203160 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DGST2 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DEPT1 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DRAB4 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DHMGB This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DALG2 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DDGAT1 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DTGGT1_211695 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DPDX1 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DPDX2 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DEF-P This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DTGGT1_204350 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DTGGT1_215890 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DUSPase This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DRad23 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DHID1 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DSui1 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DGRA17 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DGRA72+ GRA72-HA This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/ GRA23-HA This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DGRA72/GRA23-HA This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DUFM1 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DUFM1+UFM1(WT) This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DUFM1+UFM1(G87A) This study N/A C57BL/6 mice SLC N/A Ifngr1 / mice Sasai et al.71 N/A Oligonucleotides For primers and oligonucleotides, see Table S4 This study N/A Recombinant DNA For each gRNA sublibrary, see Tables S1–S3 This study N/A pU6-Universal Sidik et al.72 Addgene plasmid: #52694 pSAG1-GRA72-HA-HXGPRT This study N/A pUPRT-UFM1(WT) This study N/A pUPRT-UFM1(G87A) This study N/A (Continued on next page) Cell Reports 42, 112592, June 27, 2023 17

    Techniques: In Vivo, In Vitro, Residue, Infection